Complement C3 (C3) Rabbit Polyclonal Antibody

CAT#: AP10423PU-N

Complement C3 (C3) rabbit polyclonal antibody, Purified

Product Images

Formalin-Fixed, Paraffin-Embedded Human transplanted kidney stained with C3d antibody Cat.-No. AP10423PU-N using peroxidase conjugate and DAB chromogen. Note basement membrane staining of renal tubules and glomeruli.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Formalin-Fixed, Paraffin-Embedded Sections: 1/50-1/200
  Pretreatment of deperaffinized tissue with heat-induced epitope retrieval is recommended.
  Use Polymer anti Mouse/Rabbit IgG as a detection system.
  Positive Control: Acute rejected kidney transplant.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to C3d
• Specificity: Recognizes Complement C3d.
  Cellular Localization: Membrane or cytoplasmic.
• Formulation: State: Purified
  State: Liquid purified ig fraction with 0.2% BSA and 15mM Sodium Azide.
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens B-cell CLL/lymphoma 6 (BCL6), transcript variant 2
• Database Link:
  Entrez Gene 604 HumanEntrez Gene 718 Human
• Background: Complement component C3 plays a central role in the activation of complement system. Its activation is required for both classical and alternative complement activation pathways. C3d deposition in the renal transplant PTCs (peritubular capillaries) is indicative of AR (acute rejection) with subsequent high probability of graft loss. Anti-C3d, combined with anti-C4d, can be utilized as a tool for diagnosis of AR and warrant prompt and aggressive anti-rejection treatment. It was shown that anti-C3d labeled the epidermal basement membrane in 97% (31/32) cases of bullous pemphigoid (BP), with none of the normal controls demonstrating such findings. In the same study 27% (3/11) cases of pemphigus vulgaris (PV) demonstrated intercellular C3d deposition. Therefore, C3d immunohistochemistry is a helpful adjunct in the diagnosis of BP (and perhaps PV), especially in the cases in which only formalin-fixed, paraffin embedded tissue is available for analysis.
• Synonyms: CPAMD1, Complement component 3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors