Pumilio 1 (PUM1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP14499PU-N

Pumilio 1 (PUM1) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Western blot analysis of PUM1 antibody (Human, N-term) in HL-60 cell line lysates (35 ug/lane). PUM1 (arrow) was detected using the purified Pab (2 ug/ml)

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  Figure 2. Formalin-fixed and paraffin-embedded Human testis tissue reacted with PUM1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for Immunohistochemistry. Clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/1,000.
  Western blot: 1/1,000.
  Immunohistochemistry: 1/10~1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human PUM1.
• Specificity: This antibody recognizes Pumilio 1 (PUM1).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein A Chromatography followed by peptide affinity purification.
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens pumilio RNA binding family member 1 (PUM1), transcript variant 2
• Database Link:
  Entrez Gene 9698 Human
• Background: PUM1 is a sequence-specific RNA-binding protein that regulates translation and mRNA stability by binding the 3'-UTR of mRNA targets. Its interactions and tissue specificity suggest that it may be required to support proliferation and self-renewal of stem cells by regulating the translation of key transcripts.
• Synonyms: Pumilio homolog 1, HsPUM, Pumilio-1, PUMH1, KIAA0099
• Note: Calculated Molecular Weight: 126473 Da