KIF4A (C-term) Goat Polyclonal Antibody

CAT#: AP16036PU-N

KIF4A (C-term) goat polyclonal antibody, Aff - Purified


USD 375.00

5 Days*

Size
    • 100 ug

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Specifications

Product Data
Applications FC, IF, WB
Recommended Dilution Peptide ELISA: 1/64000 (Detection Limit).  
Western blot: 1-2 µg/ml.  Approx 150kDa band observed in nuclear lysates of cell line NIH3T3 (calculated MW of 139.5kDa according to Mouse NP_032472.2).
Primary incubation: 1 hour at room temperature.
Negative Control: Human Ovary lysate. 
Immunofluorescence: 10 µg/ml. Strong expression of the protein seen in the nucleus of U2OS cells. 
Flow Cytometry analysis of HEK293 cell: 10µg/ml.
Reactivities Bovine, Human, Mouse, Porcine, Rat
Host Goat
Clonality Polyclonal
Immunogen Synthetic peptide from the C-Terminus of Human KIF4A (NP_036442.3)
Specificity This antibody detects KIF4A at C-term.
Formulation Tris saline, pH~7.3
State: Aff - Purified
State: Liquid purified Ig fraction
Stabilizer: 0.5% BSA
Preservative: 0.02% Sodium Azide
Concentration 0.5 mg/ml
Purification Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
Background Kinesins, such as KIF4A are required for mitotic chromosomal positioning and bipolar spindle stabilization. It is located in the nucleus and is associated with mitotic chromosomes. It is highly expressed in hematopoietic tissues, fetal liver, spleen, thymus and adult thymus and bone marrow. Lower levels are found in heart, testis, kidney, colon and lung.
Synonyms KIF4A, KIF4, Chromosome-associated kinesin KIF4A, Chromokinesin-A
Reference Data

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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.