ERG (C-term) Goat Polyclonal Antibody

CAT#: AP16447PU-N

ERG (C-term) goat polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry: (4ug/ml) staining of paraffin embedded Human Prostate. Steamed antigen retrieval with Tris/EDTA buffer pH 9, HRP-staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Peptide ELISA: 1/16000 (Detection Limit).
  Western blot: Approx 55kDa band observed in lysates of cell line MOLT4 (calculated MW of 53.8kDa according to NP_891548.1).
  Recommended concentration: 0.1-0.3 µg/ml.
  Primary incubation was 1 hour.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from the C-Terminus of Human ERG (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1)
• Specificity: This antibody is expected to recognize all reported isoforms (NP_891548.1; NP_004440.1; NP_001129626.1; NP_001129627.1; NP_001230358.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  Entrez Gene 2078 Human
• Background: ERG (v-ets erythroblastosis virus E26 oncogene like isoform 2) is a transcriptional regulator. It may participate in transcriptional regulation through the recruitment of SETDB1 histone methyltransferase and subsequent modification of local chromatin structure.
• Synonyms: Transcriptional regulator ERG, Transforming protein ERG