BCKDHA (C-term) Rabbit Polyclonal Antibody

CAT#: AP17149PU-N

BCKDHA (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of BCKDHA Antibody (C-term) in Jurkat cell line lysates (35 ug/lane). BCKDHA (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blot: 1:1000.
  ELISA: 1:1,000.
  FLow Cytometry: 1/10-50.
  Immunohistochemistry: 1/50-100.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected between aa 362-390 of the C-terminal region of human BCKDHA
• Specificity: This antibody detects BCKDHA at C-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens branched chain keto acid dehydrogenase E1, alpha polypeptide (BCKDHA), transcript variant 1
• Database Link:
  Entrez Gene 593 Human
• Background: The branched-chain alpha-keto acid (BCAA) dehydrogenase(BCKD) complex is an innter mitochondrial enzyme complex that catalyzes the second major step in the catabolism of the branched-chain amino acids leucine, isoleucine, and valine. The BCKD complex consists of three catalytic components: a heterotetrameric (alpha2-beta2) branched-chain alpha-keto acid decarboxylase (E1), a dihydrolipoyl transacylase (E2), and a dihydrolipoamide dehydrogenase (E3). BCKDHA is the alpha subunit of the decarboxylase (E1) component.
• Synonyms: alpha-keto acid dehydrogenase, BCKDE1A
• Note: Molecular weight: 50471 Da

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Valine, leucine and isoleucine degradation