ERAB (HSD17B10) Rabbit Polyclonal Antibody

CAT#: AP20285PU-N

ERAB (HSD17B10) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of HADH2 antibody (AP20285PU-N) in extracts from LOVO cells.

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  Immunohistochemistry analyzes of HADH2 antibody (AP20285PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of HADH2 protein.
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 27 kDa
• Gene Name: Homo sapiens hydroxysteroid 17-beta dehydrogenase 10 (HSD17B10), transcript variant 2
• Database Link:
  Entrez Gene 15108 MouseEntrez Gene 63864 RatEntrez Gene 3028 Human
  UniProt ID Q99714
• Background: β-Amyloid is a neurotoxic peptide that is associated with the pathogenesis of Alzheimer’s disease. β-Amyloid aggregates induce cell death of neurons through the disruption of cell membranes and the generation of reactive oxygen intermediates.These neurotoxic effects are also attributed to the interaction of β-Amyloid with intracellular proteins, specifically ERAB, the endoplasmic reticulum-associated β-Amyloid-binding protein. ERAB is characterized as a NAD+-dependent dehydrogenase that is constitutively expressed in tissues and overexpressed in neurons affected in Alzheimer’s disease. Cells overexpressing ERAB in vitro have been shown to be more sensitive to β-Amyloid-induced stress, and blocking the activity of ERAB has been shown to inhibit this cell death, indicating that β-Amyloid induced cell death is mediated by ERAB.
• Synonyms: HADH2, MRPP2, SCHAD, XH98G2, 17-beta-HSD10, Mitochondrial Marker

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Metabolic pathways, Valine, leucine and isoleucine degradation