Aryl hydrocarbon Receptor (AHR) Rabbit Polyclonal Antibody

CAT#: AP20330PU-N

Aryl hydrocarbon Receptor (AHR) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry analysis of AhR antibody (Cat.-No AP20330PU-N) in paraffin-Embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western Blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of AhR protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~96 kDa
• Gene Name: Homo sapiens aryl hydrocarbon receptor (AHR)
• Database Link:
  Entrez Gene 11622 MouseEntrez Gene 25690 RatEntrez Gene 196 Human
  UniProt ID P35869
• Background: The Aryl Hydrocarbon Receptor (AHR), also known as the dioxin receptor, is a ligand-activated helix/loop/helix transcription factor found in a variety of vertebrate species. The known ligands for AHR are foreign planar aromatic compounds, such as polycyclic aromatic compounds and halogenated aromatic compounds such as 2,3,7,8-TetraChloroDibenzo-p-Dioxin (TCDD). Unlike the steroid/thyroid hormone receptors, there is no known physiological ligand for the AH Receptor.
  Studies indicate that in non-ligand activated cells, AHR is found complexed with HSP90 predominantly in the cytoplasm. Upon binding to an agonist, the ligand-activated AHR is believed to transform to a nuclear, DNA binding form. This transformation process appears to involve dissociation of HSP90 from AHR followed by formation of a heterodimer with AHR nuclear translocator protein (Arnt).
  The AHR-ligand complex appears to initiate gene transcription of cytochrome P450 1A1.
• Synonyms: AHR, BHLHE76, Aryl hydrocarbon receptor

Reference Data

• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors