LIM Kinase 1 (LIMK1) Rabbit Polyclonal Antibody

CAT#: AP20386PU-N

LIM Kinase 1 (LIMK1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IMK-1 antibody (Cat.-No.: AP20386PU-N) in extracts from mouse brain.

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  Immunohistochemistry (IHC) analyzes of IMK-1 antibody (Cat.-No.: AP20386PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of LIMK-1 protein.
  (region surrounding Gln491)
• Formulation: PBS, pH 7.2 containing 0.05% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purfied Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 72 kDa
• Gene Name: Homo sapiens LIM domain kinase 1 (LIMK1), transcript variant 1
• Database Link:
  Entrez Gene 16885 MouseEntrez Gene 65172 RatEntrez Gene 3984 Human
• Background: There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase 1 and LIM kinase 2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a C-terminal protein kinase domain. LIMK1 is likely to be a component of an intracellular signaling pathway and may be involved in brain development. LIMK1 hemizygosity is implicated in the impaired visuospatial constructive cognition of Williams syndrome. Two splice variant have been identified.
• Synonyms: LIMK, LIM domain kinase 1, LIMK-1, EC=2.7.11.1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton