GTF2A1 (TFIIA-alpha) Rabbit Polyclonal Antibody

CAT#: AP20422PU-N

GTF2A1 (TFIIA-alpha) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of TFIIA-a antibody (Cat.-No.: AP20422PU-N) in extracts from RAW264.7 cells.

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  Immunohistochemistry analyzes of TFIIA-a antibody (Cat.-No.: AP20422PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunohistochemistry on paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of TFIIA-α protein.
  (region surrounding Glu311)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 42 kDa
• Gene Name: Homo sapiens general transcription factor IIA subunit 1 (GTF2A1), transcript variant 1
• Database Link:
  Entrez Gene 83602 MouseEntrez Gene 83830 RatEntrez Gene 2957 Human
  UniProt ID P52655
• Background: Initiation of transcription from protein-coding genes in eukaryotes is a complex process that requires RNA polymerase II, as well as families of basal transcription factors. Binding of the factor TFIID (TBP) to the TATA box is believed to be the first step in the formation of a multiprotein complex containing several additional factors, including TFIIA, TFIIB, TFIIE, TFIIF and TFIIH. Recognition of the TATA binding element by TBP, one of the first steps in transcription initiation, may be regulated by TFIIA. TFIIA consists of three subunits designated TFIIA-α, TFIIA-β and TFIIA-γ, and it interacts with both TBP and a TAF (TBP-associated factor). It has been demonstrated that the basic region of TBP is essential for TFIIA-dependent function of TBP.
• Synonyms: TFIIA-42, TFIIAL

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors