MELK Rabbit Polyclonal Antibody

CAT#: AP20443PU-N

MELK rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of MELK antibody (Cat.-No.: AP20443PU-N) in extracts from k562 cells.

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  Immunohistochemistry (IHC) analyzes of MELK antibody (Cat.-No.: AP20443PU-N) in paraffin-embedded human liver carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MELK protein.
  (region surrounding asn449)
• Formulation: Phosphate buffered saline (PBS), pH~7.2 with 0.05% Sodium Azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~75 kDa
• Gene Name: Homo sapiens maternal embryonic leucine zipper kinase (MELK), transcript variant 1
• Database Link:
  Entrez Gene 9833 Human
  UniProt ID Q14680
• Background: Maternal Embryonic Leucine Zipper Kinase (KIAA0175, HPK38) or MELK, a new member of the Snf1/AMPK family of kinases, encodes a protein with a kinase catalytic domain and a leucine zipper motif consisting of a periodic repetition of leucine residues at every seventh residue located within the N-terminal catalytic domain. This motif has been observed in myriad DNAbinding proteins and is presumed to be involved in protein-DNA interactions, and potentially protein-protein interactions. Research predicts that the gene product of MELK plays a role in the signal transduction events in the egg and early embryo. Mouse and human MELK proteins share 95% sequence identity in the kinase domain and northern blot analysis in mouse indicates that MELK expression is restricted to spermatogonia in the testis and to oocytes in the ovary.
• Synonyms: Maternal embryonic leucine zipper kinase, KIAA0175, hMELK

Reference Data

• Protein Families: Druggable Genome, Protein Kinase