SIAH1 (+SIAH2) Rabbit Polyclonal Antibody

CAT#: AP20448PU-N

SIAH1 (+SIAH2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Siah-1/2 Antibody (Cat.-No AP20448PU-N) in extracts from HeLa cells at 1/500 dilution.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Siah-1/2 protein.
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 15 mM Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~40.0 kDa
• Gene Name: Homo sapiens siah E3 ubiquitin protein ligase 1 (SIAH1), transcript variant 2
• Database Link:
  Entrez Gene 140941 RatEntrez Gene 6477 Human
• Background: SIAH, the human homologue of the Drosophila seven in absentia (sina) gene, is a tumor suppressor protein that is expressed in intestinal epithelium and activated during apoptosis. Human SIAH proteins are produced as two distinct gene products, SIAH-1, and a slightly larger protein SIAH-2, which share a highly conserved C-terminal sequence and differ in their N-terminal regions. SIAH-1 is a 32 kDa protein that contains an N-terminal RING-finger domain, which is required for proteolysis, and a cystein-rich C-terminal domain, which regulates oligomerization and SIAH binding to target proteins. As a tumor suppressor, SIAH-1 binds DCC (deleted in colorectal cancer) and regulates DCC degradation via the ubiquitin-proteasome pathway. SIAH-1 also binds a Bcl-2 related protein, Bag-1, thereby inhibiting cell growth. The majority of SIAH-1 is localized to the nucleus, however a small percentage is detected in the cytoplasm. This nuclear localization suggests that SIAH proteins may interact with other nuclear matrix proteins and DNA.
• Synonyms: Siah-1, Siah-1a, HUMSIAH

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: p53 signaling pathway, Ubiquitin mediated proteolysis, Wnt signaling pathway