BUB3 Rabbit Polyclonal Antibody

CAT#: AP20475PU-N

BUB3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of Bub3 antibody (Cat.-No.: AP20475PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of Bub3 antibody (Cat.-No.: AP20475PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 251-300 of Human BUB3.
• Specificity: This antibody detects endogenous levels of Bub3 protein.
  (region surrounding His274)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction ( > 95% pure by SDS-PAGE)
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 40 kDa
• Gene Name: Homo sapiens BUB3, mitotic checkpoint protein (BUB3), transcript variant 2
• Database Link:
  Entrez Gene 12237 MouseEntrez Gene 361662 RatEntrez Gene 9184 Human
  UniProt ID O43684
• Background: The mitotic checkpoint protein Bub3 appears to be essential during normal mitotic progression, preventing premature sister chromatid separation, missegreation and aneuploidy. Bub3 is also important during G2 and early mitosis stages, promoting normal mitotic entry. Loss of Bub3 function causes slower progression through prophase, apparently due to mitotic cyclins A and B not being accumulated.
• Synonyms: BUB3L; hBUB3

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Cell cycle