GEF H1 (ARHGEF2) Rabbit Polyclonal Antibody

CAT#: AP20505PU-N

GEF H1 (ARHGEF2) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of ARHGEF2 antibody (Cat.-No.: AP20505PU-N) in extracts from NIH cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of ARHGEF2 protein.
  (region surrounding Pro881)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 110 kDa
• Gene Name: Homo sapiens Rho/Rac guanine nucleotide exchange factor 2 (ARHGEF2), transcript variant 3
• Database Link:
  Entrez Gene 16800 MouseEntrez Gene 310635 RatEntrez Gene 9181 Human
  UniProt ID Q92974
• Background: GEFs catalyze the release of GDP from inactive Ras proteins to ena-ble GTP binding. Lfc (also designated Rho/Rac guanine nucleotide exchange factor 2, lymphoid blast crisis-like 1, ARHGEF2, GEF-H1, KIAA0651 or LFP40) is an 894 amino acid GEF for Rho A, a member of the Ras family that regulates the Actin cytoskeleton during the formation of stress fibers. Lfc directly interacts with Cingulin, a junctional adapter that inhibits Rho A, thus preventing phorbol-12-myristate-13-acetate-induced contraction and apoptosis. Mutations in the p53 gene transcriptionally activate Lfc, thereby increasing tumor cell proliferation.
• Synonyms: KIAA0651, LFP40, GEF-H1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection