Cytokeratin 14 (KRT14) Rabbit Polyclonal Antibody

CAT#: AP20622PU-N

Cytokeratin 14 (KRT14) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry analyzes of Cytokeratin 14 antibody (Cat.-No.: AP20622PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This detects endogenous levels of Cytokeratin 14 protein. (region surrounding Tyr151)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 52 kDa
• Gene Name: Homo sapiens keratin 14 (KRT14)
• Database Link:
  Entrez Gene 16664 MouseEntrez Gene 287701 RatEntrez Gene 3861 Human
• Background: Cytokeratins comprise a diverse group of intermediate filament proteins (IFPs) that are expressed in pairs in both keratinized and non-keratinized epithelial tissue, where they constitute up to 85% of mature keratinocytes in the vertebrate epidermis. Cytokeratins play a critical role in differentiation and tissue specialization and function to maintain the overall structural integrity of epithelial cells. The alpha-helical coiled-coil dimers associate laterally end-to-end to form 10-nm diameter filaments. Cytokeratins are useful markers of tissue differentiation, and in addition they aid in the characterization of malignant tumors. In Bowen’s disease, the characteristic malignancy of the epidermis exhibits distinct expression patterns of cytokeratin 14. The gene encoding human cytokeratin 14 maps to chromosome 17q12- 21. Mutations in this gene lead to epidermolysis bullosa simplex, an inheritied skin disorder characterized by skin blistering due to basal keratinocyte fragility.
• Synonyms: Cytokeratin-14, CK14, Keratin 14, K14, KRT14