RPS6 Rabbit Polyclonal Antibody

CAT#: AP20711PU-N

RPS6 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of Ribosomal Ribosomal Protein S6 antibody (Cat.-No.: AP20711PU-N) in extracts from hela cells.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Ribosomal Protein S6 protein.
  (region surrounding Ala229)
• Formulation: Phosphate buffered saline (PBS), pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 15 mM sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity-chromatography using epitope-specific immunogen; purity is > 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 32 kDa
• Gene Name: Homo sapiens ribosomal protein S6 (RPS6)
• Database Link:
  Entrez Gene 20104 MouseEntrez Gene 29304 RatEntrez Gene 6194 Human
  UniProt ID P62753
• Background: Ribosomal protein S6 is a component of the small 40S ribosomeal subunit and belongs to the S6E family of ribosomal proteins. It is the major substrate of protein kinases in the ribosome, with subsets of five C-terminal serine residues phosphorylated by different protein kinases. Phosphorylation is induced by a wide range of stimuli, including growth factors, tumor-promoting agents, and mitogens. Dephosphorylation occurs at growth arrest. The protein may contribute to the control of cell growth and proliferation through the selective translation of particular classes of mRNA. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
• Synonyms: Phosphoprotein NP33, 40S ribosomal protein S6

Reference Data

• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway, Ribosome