PKR (EIF2AK2) Rabbit Polyclonal Antibody

CAT#: AP20747PU-N

PKR (EIF2AK2) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyzes of PKR antibody (Cat.-No.: AP20747PU-N) in paraffin-embedded human colon carcinoma tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PKR protein.
  (region surrounding Arg445)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 74 kDa
• Gene Name: Homo sapiens eukaryotic translation initiation factor 2 alpha kinase 2 (EIF2AK2), transcript variant 2
• Database Link:
  Entrez Gene 5610 Human
• Background: An interferon-inducible, RNA-dependent protein serine/threonine kinase (PKR) has been described. PKR in earlier literature is variously known as DAI, dsJ, PI kinase, p65, p67 or TIK for the mouse kinase; and p68 or p69 for the human kinase. The PKR kinase substrate is the α subunit of protein synthesis initiation factor eIF-2. Phosphorylation of eIF-2alpha on serine-51 results in inhibition of translation. Molecular cDNA clones have been isolated from both human and mouse cells. The serine/threonine kinase catalytic domains map to the carboxy terminal half of the protein while the RNA-binding domains are located in the amino terminal region. Three kinds of regulation of PKR enzymatic activity have been described. These include transcriptional regulation in response to interferon, an autoregulatory mechanism controlling PKR expression at the level of translation and post-translational regulation by RNA mediated autophosphorylation.
• Synonyms: eIF-2A protein kinase 2, PRKR

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors