TRAF6 Rabbit Polyclonal Antibody

CAT#: AP20777PU-N

TRAF6 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of TRAF6 antibody (Cat.-No.: AP20777PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of TRAF6 antibody (Cat.-No.: AP20777PU-N) in paraffin-embedded human testis tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Mfn2 protein.
  (region surrounding His154)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 58 kDa
• Gene Name: Homo sapiens TNF receptor associated factor 6 (TRAF6), transcript variant 2
• Database Link:
  Entrez Gene 22034 MouseEntrez Gene 311245 RatEntrez Gene 7189 Human
  UniProt ID Q9Y4K3
• Background: Tumor necrosis factor receptor-associated factor 6 (TRAF6) regulates adaptive immunity, innate immunity and bone metabolism. TRAF6 is a ubiquitin (Ub) ligase that mediates the activation of protein kinases, such as transforming growth factor beta-activated kinase (TAK1) and IkappaB kinase (IKK), by catalyzing the formation of a unique polyubiquitin chain linked through Lys 63 of Ub. TRAF6 is essential for activating NFκB signaling pathway in response to interleukin-1 and Toll-like receptor ligands. The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination and activating NFkappaB signaling pathway. TRAF6 interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways.
• Synonyms: TNF receptor-associated factor 6

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway, Ubiquitin mediated proteolysis