Neurofilament (NEFL) Rabbit Polyclonal Antibody

CAT#: AP20786PU-N

Neurofilament (NEFL) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry analysis of NF-L in human lung carcinoma tissue (Formalin-fixed, Paraffin-embedded) using Neurofilament L antibody (Cat.-No. AP20786PU-N).

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on Paraffin sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of NF-L protein.
  (region surrounding Ile344)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% Sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage:

  Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Predicted Protein Size: ~ 62 kDa
• Gene Name: Homo sapiens neurofilament, light polypeptide (NEFL)
• Database Link:
  Entrez Gene 18039 MouseEntrez Gene 83613 RatEntrez Gene 4747 Human
• Background: Neurofilament-L (for neurofilament light polypeptide, or NF-L), a member of the intermediate filament family, is a major component of neuronal cytoskeletons. Neurofilaments are dynamic structures; they contain phosphorylation sites for a large number of protein kinases, including protein kinase A, protein kinase C, cyclin-dependent kinase 5, extracellular signal regulated kinase, glycogen synthase kinase-3, and stress-activated protein kinase gamma. In addition to their role in the control of axon caliber, neurofilaments may affect other cytoskeletal elements, such as microtubules and actin filaments. Changes in neurofilament phosphorylation or metabolism are frequently observed in neurodegenerative diseases, including amyotrophic lateral sclerosis (ALS),Parkinson's disease, and Alzheimer's disease.
• Synonyms: NF-L,NEFL, NF68, 68 kDa neurofilament protein

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS
• Protein Pathways: Amyotrophic lateral sclerosis (ALS)