Cofilin 1 (CFL1) pSer3 Rabbit Polyclonal Antibody

CAT#: AP20808PU-N

Cofilin 1 (CFL1) pSer3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-Cofilin antbody (Cat.-No.: AP20808PU-N) in extracts from 3T3 paclitaxel cells.

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  Immunohistochemistry (IHC) analyzes of p-Cofilin (Cat.-No.: AP20808PU-N) in paraffin-embedded human lung carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of Cofilin protein only when phosphorylated at Ser3.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 19 kDa
• Gene Name: Homo sapiens cofilin 1 (CFL1)
• Database Link:
  Entrez Gene 12631 MouseEntrez Gene 29271 RatEntrez Gene 1072 Human
  UniProt ID P23528
• Background: Cofilin is ubiquitously expressed in eukaryotic cells where it binds to Actin, thereby regulating the rapid cycling of Actin assembly and disassembly, essential for cellular viability. Cofilin is a low molecular weight protein that binds to filamentous F-Actin by bridging two longitudinally-associated Actin subunits, changing the F-Actin filament twist. This process is allowed by the dephosphorylation of Cofilin Ser 3 by factors like opsonized zymosan. LIM kinase 1 (LIMK-1), a serine kinase, phosphorylates Cofilin and renders it unable to bind and depolymerize F-Actin.
• Synonyms: CFL1, CFL, p18

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Axon guidance, Fc gamma R-mediated phagocytosis, Regulation of actin cytoskeleton