HSF1 pSer303 Rabbit Polyclonal Antibody

CAT#: AP20867PU-N

HSF1 pSer303 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-HSF1 antibody (Cat.-No.: AP20867PU-N) in extracts from A549 PMA cells.

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  Immunohistochemistry (IHC) analyzes of p-HSF1 antibody (Cat.-No.: AP20867PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-HSF1 protein.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Predicted Protein Size: ~ 82 kDa
• Gene Name: Homo sapiens heat shock transcription factor 1 (HSF1)
• Database Link:
  Entrez Gene 15499 MouseEntrez Gene 3297 Human
• Background: Prokaryotic and eukaryotic cells respond to thermal and chemical stress by inducing a group of genes collectively designated heat shock genes. In eukaryotes, this gene expression is regulated primarily at the transcription level. Heat shock transcription factors (HSF, also designated HSTF) 1 and 2 are involved in this regulation. HSF1 and HSF2 are upregulated by estrogen at both the mRNA and protein level. HSF1 is normally found as a monomer whose transcriptional activity is repressed by constitutive phosphorylation. Upon activation, HSF1 forms trimers, gains DNA binding activity and is translocated to the nucleus. HSF2 activity is associated with differentiation and development and, like HSF1, binds DNA as a trimer. Both HSF1 and HSF2 are known to be induced by proteasome inhibitors of the ubiquitin pathway. Phosphorylation of HSF1 on Ser 230 by heat shock has been shown to positively contribute to the transcriptional activity of HSF1.
• Synonyms: HSTF1, HSF-1

Reference Data

• Protein Families: Transcription Factors