PDGF Receptor beta (PDGFRB) Rabbit Polyclonal Antibody

CAT#: AP20991PU-N

PDGF Receptor beta (PDGFRB) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB)analyzes of PDGFR beta antibody (Cat.-No.: AP20991PU-N) in extracts from NIH/3T3 cells.

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  Immunohistochemistry (IHC) analyzes of PDGFR beta antibody (Cat.-No.: AP20991PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PDGFR beta protein.
  (region surrounding Asp757)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 124, 190 kDa
• Gene Name: Homo sapiens platelet derived growth factor receptor beta (PDGFRB)
• Database Link:
  Entrez Gene 18596 MouseEntrez Gene 24629 RatEntrez Gene 5159 Human
• Background: Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and gliaderived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms, PDGF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-alpha and -beta, which are endowed with protein tyrosine kinase domains. PDGFR-alpha can bind to both A and B subunits of PDGF, while PDGFR-beta can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two alpha receptors, PDGF-AB induces dimerization of alpha-alpha and alpha-beta and PDGF-BB induces the formation of three types of dimers, alpha-alpha, alpha-beta and beta-beta. The genes encoding PDGFR-alpha and -beta map to human chromosome 4q11-q13 and 5q31-32, respectively. The expressed alpha and beta receptor proteins migrate at 170 and 180 kDa, respectively, by SDSPAGE. Translocation of the PDGFR-beta gene with the Tel gene is linked to chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors.
• Synonyms: PDGF-R-beta, PDGF Receptor beta

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane
• Protein Pathways: Calcium signaling pathway, Colorectal cancer, Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton