Collagenase / ColH Rabbit Polyclonal Antibody

CAT#: AP21244AF-N

Collagenase / ColH rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of the customer's own choice.
  Working dilutions in non-precipitating antibody-binding techniques: 1/10,000-1/100,000.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Collagenase is isolated and purified from Clostridium histolyticum.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Collagenase from Clostridium histolyticum.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized Hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography