PSMA4 Goat Polyclonal Antibody

CAT#: AP21301PU-N

PSMA4 goat polyclonal antibody, Aff - Purified

Product Images

PSMA4 antibody staining of K562 cell lysate at 1ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  PSMA4 antibody staining of NIH3T3 (A), Mouse Spleen (B), Rat Spleen (C)) lysates at 0.3 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: 1/4000 (Detection Limit).
  Western blot: 0.3-1 µg/ml. This antibody detects a band of ~28kDa in lysates of cell line K562, NIH3T3 and in lysates of Human Tonsil, Human Peripheral Blood Mononucleocytes and in Mouse and Rat Spleen.
• Reactivities: Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_002780.1 and NP_001096138.1
• Specificity: This antibody is expected to recognize both reported isoforms (NP_002780.1 and NP_001096138.1).
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens proteasome subunit alpha 4 (PSMA4), transcript variant 2
• Database Link:
  Entrez Gene 26441 MouseEntrez Gene 29671 RatEntrez Gene 5685 Human
• Background: The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMA4 is a member of the peptidase T1A family, that is a 20S core alpha subunit. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: Proteasome subunit alpha type-4, Macropain subunit C9, Proteasome component C9, HC9, PSC9, Proteasome subunit L
• Note: Calculated Molecular Weight: 29.5kDa (NP_002780.1)

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Proteasome