Luciferase Rabbit Polyclonal Antibody

CAT#: AP21341BT-N

Luciferase rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques (1/1,000-1/10,000).
• Reactivities: Photinus pyralis
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Luciferase isolated and purified from Photinus pyralis.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins.
  Label: Biotin
  State: Lyophilized purified IgG fraction.
  Molar radio: Biotin/IgG: ~1.7
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water.
• Concentration: 10 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
• Conjugation: Biotin
• Background: Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.