NP Rabbit Polyclonal Antibody

CAT#: AP21354BT-N

NP rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/20,000.
• Reactivities: Bovine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Nucleoside phosphorylase isolated and purified from calf spleen.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Nucleoside Phosphorylase from Calf spleen.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and enzyme inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~ 5.0
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Background: This gene encodes an enzyme which reversibly catalyzes the phosphorolysis of purine nucleosides. This enzyme, together with adenosine deaminase (ADA) serves a key role in purine catabolism. The enzyme is trimeric, containing three identical subunits. Mutations which result in nucleoside phosphorylase deficiency result in defective T-cell (cell-mediated) immunity but can also affect B-cell immunity and antibody responses. Neurologic disorders may also be apparent in patients with immune defects. A known polymorphism at aa position 51 that does not affect enzyme activity has been described. A pseudogene has been identified on chromosome 2.
• Synonyms: PNP, PNP1, Inosine Phosphorylase