phoA Rabbit Polyclonal Antibody

CAT#: AP21367BT-N

phoA rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Recommended Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/80,000.
• Reactivities: Escherichia coli
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Alkaline Phosphatase isolated and purified from Escherichia coli.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Alkaline Phosphatase from Escherichia coli.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized purified Hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~ 6.6
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Background: Alkaline phosphatase (ALP) removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes: placental, placental like, intestinal and non tissue specific (found in liver, kidney and bone). Tissues with particularly high concentrations of ALP include the liver, bile ducts, placenta, and bone. Damaged or diseased tissue releases enzymes into the blood, so serum ALP measurements can be abnormal in many conditions, including bone disease and liver disease.
• Synonyms: phoA, b0383, JW0374