Pla2 Rabbit Polyclonal Antibody

CAT#: AP21373AF-N

Pla2 rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This antibody can be used:
  In precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  To prepare an insoluble immuno-affinity adsorbent.
  For labelling with a marker of the customer’s own choice.
  Recommended Dilutions:
  non-precipitating antibody-binding assays: 1/1,000-1/80,000.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospholipase A2 isolated and purified from bee venom.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Phospholipase A2 from bee venom.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), Block Titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives.
  State: Azide Free
  State: Lyophilized purified IgG fraction.
• Reconstitution Method: Restore by adding 1 ml of sterile distilled water.
• Concentration: 10 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography.
• Background: Phospholipase A2 catalyzes the hydrolysis of the sn-2 position of membrane glycerophospholipids to liberate arachidonic acid, a precursor of eicosanoids including prostaglandins and leukotrienes.
  This enzyme has been proposed to hydrolyze phosphatidylcholine in lipoproteins to liberate lyso-PC and free fatty acids in the arterial wall, thereby facilitating the accumulation of bioactive lipids and modified lipoproteins in atherosclerotic foci.
  Phospholipase A2 IIA can exert beneficial action in the context of infectious diseases since recent studies have shown that this enzyme exhibits potent bactericidal effects. Induction of the synthesis of Phospholipase A2 IIA is generally initiated by endotoxin and a limited number of cytokines via paracrine and/or autocrine processes.
• Synonyms: Allergen Api m I, Phosphatidylcholine 2-acylhydrolase