Phospholipase C / PLC Rabbit Polyclonal Antibody

CAT#: AP21376BT-N

Phospholipase C / PLC rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
  Working dilutions: vary widely, but may be between 1/1000 - 1/20000.
• Reactivities: Bacillus sp.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospholipase C isolated and purified from Bacillus cereus. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: Biotin-conjugated IgG fraction of polyclonal rabbit antiserum to phospholipase C from Bacillus cereus.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: immunoelectrophoresis, cross-immunoelectrophoresis, single radial immunodiffusion (Ouchterlony), block titration, ELISA, immunoblotting and enzyme inhibition.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized purified IgG fraction.
  Molar radio: Biotin/IgG ~5.4
• Reconstitution Method: Restore by adding 1,0 ml of sterile distilled water.
• Concentration: 10,0 mg/ml
• Purification: Hyperimmune antisera with strong precipitating activity are selected for Fractionation by Salt-Precipitation and DEAE-Chromatography.
• Conjugation: Biotin
• Synonyms: Cereolysin A