poxB Rabbit Polyclonal Antibody

CAT#: AP21421BT-N

poxB rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working Dilutions:
  Non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
• Reactivities: Bacteria
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Pyruvate oxidase isolated and purified from bacteria. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~5.0
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: 10.0 mg/ml
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Background: Catalyzes the oxidation of pyruvate to acetylphosphate, carbon dioxide, and hydrogen peroxide in the presence of phosphate.
• Synonyms: Pyruvate dehydrogenase ubiquinone, POX