Human Kappa Light Chain (free and bound) Goat Polyclonal Antibody

CAT#: AP21453SU-N

Human Kappa Light Chain (free and bound) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used to detect and identify the light chain type of immunoglobulins or free light chains in serum or other body fluids by immunoelectrophoresis or double radial immunodiffusion.
  Recommended working dilutions:
  Immunoelectrophoresis: 2 µl serum or equivalent against 120 µl antiserum.
  Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in 3 mm diameter center well and 2 µl serum samples (neat and serially diluted in 2 mm diameter peripheral wells.
  Antibody titre: 1/32 when tested against normal human serum in agar immunodiffusion block titration.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: A pool of purified Bence Jones kappa proteins isolated from human urine. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is directed to the surface and hidden determinants of Ig kappa light chain. In immunoelectrophoresis this antiserum is reacting with polyclonal and monoclonal immunoglobulins of the kappa type, Bence Jones proteins as well as free light chains of the kappa type. This antiserum does not react with any other protein of human serum or plasma.
  Cross-reactivity: Inter-species cross-reactivity is a normal feature of antibodies to immunoglobulins and their fragments, since Ig of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail.
• Formulation: State: Serum
  State: Lyophilized (delipidated, heat inactivated) stable whole antiserum without preservatives
• Reconstitution Method: Restore by adding 1,0 ml of sterile distilled water
• Concentration: Total protein and IgG concentration in the antiserum are comparable to those of pooled normal goat serum. No foreign proteins added.
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with human serum proteins.