Lactoferrin (LTF) Goat Polyclonal Antibody

CAT#: AP21469SU-N

Lactoferrin (LTF) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: In precipitating techniques as immunoelectrophoresis, single and double radial immunodiffusion Mancini, Ouchterlony) and electroimmunodiffusion (Laurell) for the identification and quantitative determination of lactoferrin in body fluids.
  Directions for use:
  Immunoelectrophoresis: 2 µl human serum against 120 µl antiserum.
  Double radial immunodiffusion (Ouchterlony): a rosette arrangement with 10 µl antiserum in a 3 mm diameter center well and 10 µl of the antiserum in 3 mm diameter peripheral wells.
  Single radial immunodiffusion: 1% antiserum in the gel.
  Antibody titre: Precipitin titre not less than 1/32 when tested against normal human milk in agar block immunodiffusion titration.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: The immunogen has been isolated from human milk. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: In immunoelectrophoresis against human milk a single precipitin line is obtained. The antiserum does not react with any other protein component of human serum or plasma.
  Cross-reactivity: The antiserum does not cross-react with any other human plasma proteins as tested in gel-diffusion techniques. Inter-species cross-reactivity is a normal feature of antibodies to plasma or milk proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion a reaction with Rhesus monkey milk has been observed.
• Formulation: State: Serum
  State: Lyophilized, Delipidated, Heat inactivated, Stable Whole Serum without preserv
• Reconstitution Method: Restore by adding 1,0 ml of sterile distilled water
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens lactotransferrin (LTF), transcript variant 1
• Database Link:
  Entrez Gene 4057 Human
• Background: Exocrine organs produce various secretions, each with its characteristic function. Proteins found in secretions may be divided into two groups: those specific for the particular secretion, and plasma proteins independent of the type of exocrine cells. Lactoferrin belongs to the first group. It is an iron containing protein with a molecular weight of 75,000 and it is antigenically different from transferrin. Lactoferrin has a slight antimicrobal action. Originally identified in milk, its presence has also been demonstrated in other secretions as saliva, semen and tears.
• Synonyms: LTF, LF, Lactoferrin, EC=3.4.21, Talalactoferrin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized fractions of human serum and lactoferrin–depleted human milk as required, to eliminate antibodies reacting with other human serum or milk proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Druggable Genome, Protease, Secreted Protein