Transferrin (TF) Goat Polyclonal Antibody

CAT#: AP21478SU-N

Transferrin (TF) goat polyclonal antibody, Serum

Specifications

Product Data

• Applications: ID, IP
• Recommended Dilution: Can be used in Precipitating techniques as Immunoelectrophoresis and single and double radial Immunodiffusion to identify the presence transferrin in human serum or other body fluids and to determine its concentration. This antiserum has not been tested in more sensitive assays as nephelometry, ELISA or use in immunohistology, but this does not exclude such use if proper controls are included.
  Recommended dilutions:
  Immunoelectrophoresis: use 2 µl serum or equivalent against 120 µl antiserum.
  Double radial immunodiffusion: use a rosette arrangement with 10 µl antiserum in 3 mm diameter centre well and 2 µl serum samples (neat and serially diluted) in 2 mm diameter peripheral wells.
  Antibody titre Precipitin titre 1/32 when tested against pooled normal human serum in agar-block immunodiffusion titration.
• Reactivities: Human
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Purified transferrin isolated from pooled human serum. Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reactivity of the antiserum is restricted to transferrin as tested in immunoelectrophoresis and radial immunodiffusion. A characteristic single precipitin line is obtained with normal serum which shows a reaction of identity with the precipitin line with purified transferrin.
  Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to serum proteins, since homologous proteins of different species frequently share antigenic determinants. Crossreactivity of this antiserum has not been tested in detail, however in immunoelectrophoresis a weak reaction has been observed with rat and rabbit.
• Formulation: State: Serum
  State: Delipidated, heat inactivated, lyophilized, stable whole antiserum with no preservatives
• Reconstitution Method: Restore with 1 ml sterile distilled water.
• Concentration: lot specific
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens transferrin (TF)
• Database Link:
  Entrez Gene 7018 Human
• Synonyms: Serotransferrin, Siderophilin
• Note: Adsorption: Immunoaffinity adsorbed using insolubilized antigens as required, to eliminate antibodies reacting with other serum proteins. The use of insolubilized adsorption antigens prevents the presence of excess adsorbent protein or immune complexes in the antiserum.

Reference Data

• Protein Families: Druggable Genome, Secreted Protein