VCAM1 Rabbit Polyclonal Antibody

CAT#: AP22317BT-S

VCAM1 rabbit polyclonal antibody, Biotin

Product Images

Direct ELISA using CD106 / VCAM1 Antibody Cat.-No AP22317BT

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  Sandwich ELISA using CD106 / VCAM1 Antibody Cat.-No AP22317BT

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution:

  Direct ELISA: To detect Human VCAM-1 by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant Human VCAM-1.
  Sandwich ELISA: To detect Human VCAM-1 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This antigen Biotin purified antibody, in conjunction with Affinity Anti-Human VCAM-1 (cat.-No AP22317PU-N or AP22317PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of ecombinant Human VCAM-1.
  Western Blot: To detect Human VCAM-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human VCAM-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure HEK293 cells derived Recombinant Human CD106 / VCAM1
• Specificity: Recognizes Human CD106 / VCAM1.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fractionin
• Reconstitution Method:
  Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage:

  Prior to reconstitution store at 2-8°C.
  Following reconstitution store undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.

• Stability: Shelf life: one year from despatch.

• Gene Name: Homo sapiens vascular cell adhesion molecule 1 (VCAM1), transcript variant 1
• Database Link:
  Entrez Gene 7412 Human
• Background:

  CD106 (also known as vascular cell adhesion molecule-1 (VCAM-1) and INCAM-110) is a member of the Ig superfamily of adhesion molecules and is expressed at high levels on cytokine stimulated vascular endothelial cells, and at minimal levels on unstimulated endothelial cells. It is also present on follicular and interfollicular dendritic cells of lymph nodes, myoblasts, and some macrophages. CD106 serves as a ligand for leukocyte integrin alphaBeta (VLA-4 or CD49d/CD29) and mediates cell adhesion of leukocytes to activated endothelium. It plays a role in tumor metastasis.

• Synonyms: V-CAM 1, INCAM-100, L1CAM, VCAM-1

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Cell adhesion molecules (CAMs), Leukocyte transendothelial migration