P4HA1 (431-443) Goat Polyclonal Antibody

CAT#: AP22559PU-N

P4HA1 (431-443) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.2 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Human Skin (formalin-fixed, paraffin-embedded) stained with P4HA1 antibody at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Spleen (formalin-fixed, paraffin-embedded) stained with P4HA1 antibody at 5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/32000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/32000, 0.1 µg/ml.
• Reactivities: Bovine, Bat, Canine, Chicken, Equine, Human, Monkey, Porcine, Rabbit
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human P4HA1
• Specificity: This antibody detects P4HA1 (Internal).
• Formulation: Tris saline buffer, pH 7.3, 0.5% BSA, 0.02% sodium azide
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens prolyl 4-hydroxylase subunit alpha 1 (P4HA1), transcript variant 2
• Database Link:
  Entrez Gene 18451 MouseEntrez Gene 64475 RatEntrez Gene 5033 Human
• Background: P4HA1 is a component of prolyl 4-hydroxylase, a key enzyme in collagen synthesis composed of two identical alpha subunits and two beta subunits. The encoded protein is one of several different types of alpha subunits and provides the major part of the catalytic site of the active enzyme. In collagen and related proteins, prolyl 4-hydroxylase catalyzes the formation of 4-hydroxyproline that is essential to the proper three-dimensional folding of newly synthesized procollagen chains.
• Synonyms: P4HA, Prolyl 4-hydroxylase subunit alpha-1, EC=1.14.11.2, 4-PH alpha-1

Reference Data

• Protein Families: Druggable Genome, P450
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways