FOXA1 (429-439) Goat Polyclonal Antibody

CAT#: AP22568PU-N

FOXA1 (429-439) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.3 µl/ml) staining of HepG2 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Breast (formalin-fixed, paraffin-embedded) stained with FOXA1 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Prostate (formalin-fixed, paraffin-embedded) stained with FOXA1 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/8000.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1/8000, 0.3 - 1 µg/ml.
• Reactivities: Bat, Bovine, Hamster, Human, Monkey, Mouse, Porcine, Rat, Xenopus, Zebrafish
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from C-term of human FOXA1
• Specificity: This antibody reacts to FOXA1 at aa 429-439.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity chromatography
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens forkhead box A1 (FOXA1)
• Database Link:
  Entrez Gene 15375 MouseEntrez Gene 25098 RatEntrez Gene 3169 Human
• Background: Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity).
• Synonyms: Forkhead box protein A1, HNF3A, HNF-3A, HNF-3-alpha, TCF-3A

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transcription Factors