MBD2 (15-29) Goat Polyclonal Antibody

CAT#: AP22575PU-N

MBD2 (15-29) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (0.3 ug/ml) staining of Jurkat cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• 

  Human Adrenal (formalin-fixed, paraffin-embedded) stained with MBD2 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Colon (formalin-fixed, paraffin-embedded) stained with MBD2 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

• 

  Human Skin (formalin-fixed, paraffin-embedded) stained with MBD2 antibody followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/64000.
  Immunohistochemistry on Paraffin Sections: 2.5 µg/ml.
  Western Blot.
• Reactivities: Bat, Bovine, Canine, Equine, Hamster, Human, Monkey, Mouse, Porcine, Rabbit, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human MBD2
• Specificity: This antibody detects MBD2 (Internal).
  It is expected to resognize isoform 1 (NP_003918.1) only.
• Formulation: Tris saline buffer, pH 7.3 containing 0.5% BSA as stabilizer and 0.02% sodium azide as preservative
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 0.5 mg/ml
• Purification: Immunoaffinity chromatography
• Gene Name: Homo sapiens methyl-CpG binding domain protein 2 (MBD2), transcript variant 1
• Database Link:
  Entrez Gene 17191 MouseEntrez Gene 680172 RatEntrez Gene 8932 Human
• Background: DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters.
• Synonyms: Methyl-CpG-binding domain protein 2, Demethylase, DMTase

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors