ICAD (DFFA) (2-21) Rabbit Polyclonal Antibody

CAT#: AP22848PU-N

ICAD (DFFA) (2-21) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of DFF45/35 in HeLa (H), K562 (K), Jurkat (J), and Raji (R) whole cell lysate with anti-DFF45/35 (AP22848PU-N, NT) at 1/1000 dilution.

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  Human Breast (formalin-fixed, paraffin-embedded) stained with DFFA antibody AP22848PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Colon (formalin-fixed, paraffin-embedded) stained with DFFA antibody AP22848PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Pancreas (formalin-fixed, paraffin-embedded) stained with DFFA antibody AP22848PU-N at 10 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/1000 - 1/2000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: DFFA antibody was raised against synthetic peptide
• Specificity: This antibody reacts to DNA Fragmentation Factor, 45kDa, Alpha Polypeptide (DFF45/ICAD) (DFFA).
• Formulation: PBS containing 0.02% sodium azide.
  State: Purified
  State: Liquid purified Ig fraction
• Purification: DEAE-column Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens DNA fragmentation factor subunit alpha (DFFA), transcript variant 1
• Database Link:
  Entrez Gene 1676 Human
• Background: Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A human 45 kD DNA fragmentation factor (DFF45) was identified recently that was cleaved by caspase-3 during apoptosis. Mouse homologue of human DFF45 was identified as a DNase inhibitor designated ICAD. DFF45/ICAD have short forms that were termed DFF35 and ICADs, respectively. Upon cleavage of DFF45/ICAD, the caspase activated deoxyribonuclease (DFF40/CAD) is released and activated and eventually causes the degradation of DNA in the nuclei. Therefore, the cleavage of DFF45/ICAD, which causes DFF40/CAD activation and DNA degradation, is the hallmark of apoptotic cell death.
• Synonyms: DFF1, DFF45, DFF-45

Reference Data

• Protein Pathways: Apoptosis