ASK1 (MAP3K5) (N-term) Rabbit Polyclonal Antibody

CAT#: AP22867PU-N

ASK1 (MAP3K5) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of ASK1 in 3T3 cell lysate with at (A) 1 and (B) 2 ug/ml.

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  Human Heart (formalin-fixed, paraffin-embedded) stained with MAP3K5 antibody AP22867PU-N at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

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  Human Pancreas (formalin-fixed, paraffin-embedded) stained with MAP3K5 antibody AP22867PU-N at 5 ug/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA.
  Immunohistochemistry on Paraffin Sections: 5 µg/ml.
  Western Blot: 1 - 2 µg/ml.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: MAP3K5 antibody was raised against synthetic peptide - KLH conjugated
• Specificity: This antibody reacts to MAP Kinase Kinase Kinase 5 (MEKK5/ASK1) (MAP3K5).
• Formulation: PBS containing 0.02% sodium azide.
  State: Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Store the antibody undiluted at 2-8°C.
  DO NOT FREEZE!
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens mitogen-activated protein kinase kinase kinase 5 (MAP3K5)
• Database Link:
  Entrez Gene 26408 MouseEntrez Gene 4217 Human
• Background: MEKK5, also known as ASK1 for apoptosis signal-regulating kinase-1, is a MAP3K type protein kinase and a member of the JNK and p38 MAP kinase signaling pathways. The protein contains a centrally located protein kinase domain, a N-terminal extension that includes binding regions for the Fas-associated adapter protein Daxx and the redox-sensing enzyme thioredoxin, and a C-terminal extension that binds members of the TNFR-associated factor (TRAF) family. MEKK5 regulates the JNK cascade by phosphorylating MKK4/MAP2K4 and p38 cascades by phosphorylating MEK3/MAP2K3 and MKK6/MAP2K6. MEKK5 has been shown to be activated by oxidant stress, tumor necrosis factor (TNF) via TRAF2 and Fas via Daxx. MEKK5 also can be activated by Fas-Daxx in a kinase-independent apoptotic pathway.
• Synonyms: MAPK/ERK kinase kinase 5, MAPKKK5, MAP3K5

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Amyotrophic lateral sclerosis (ALS), MAPK signaling pathway, Neurotrophin signaling pathway