ACTN2 (11-347) Rabbit Polyclonal Antibody

CAT#: AP23169PU-N

ACTN2 (11-347) rabbit polyclonal antibody, Aff - Purified

Product Images

Sample (30 ug whole cell lysate). A:A431. B:H1299. C:HeLa S3. D:Hep G2. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.

• 

  Sample (30 ug of whole cell lysate). A:NIH-3T3. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.

• 

  Human Heart: Formalin-Fixed, Paraffin-Embedded (FFPE)

• 

  Human Skeletal Muscle: Formalin-Fixed, Paraffin-Embedded (FFPE)

• 

  Immunofluorescence analysis of methanol-fixed HeLa, using alpha Actinin 2 antibody at 1:200 dilution.

Show all 5 images

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Immunocytochemistry.
  Immunofluorescence: 1/100 - 1/200.
  Immunohistochemistry on Paraffin Sections: 10 µg/ml.
  Western Blot: 1/500 - 1/3000.
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant protein fragment containing a sequence corresponding to a region within amino acids 11 and 347 of alpha Actinin 2
• Specificity: This antibody detects Alpha-actinin-2 / ACTN2 at aa 11-347.
• Formulation: 0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens actinin alpha 2 (ACTN2), transcript variant 1
• Database Link:
  Entrez Gene 11472 MouseEntrez Gene 291245 RatEntrez Gene 88 Human
• Background: Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.
• Synonyms: Alpha actinin skeletal muscle isoform 2

Reference Data

• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction