Integrin alpha 4 (ITGA4) (C-term) Rabbit Polyclonal Antibody

CAT#: AP23259PU-N

Integrin alpha 4 (ITGA4) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using ITGA4 antibody

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  Immunohistochemical analysis of paraffin embedded Mammary cancer sections, staining ITGA4 in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, IP, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect ITGA4 in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect ITGA4 in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunocytochemistry: At 2-4μg/ml to detect ITGA4 in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
  Immunoprecipitation.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to the C-terminal of human ITGA4
• Specificity: This antibody detects CD49d / ITGA4 at C-term.
• Formulation: 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens integrin subunit alpha 4 (ITGA4), transcript variant 1
• Database Link:
  Entrez Gene 16401 MouseEntrez Gene 311144 RatEntrez Gene 3676 Human
• Background: The integrin family includes cell surface receptors for extracellular matrix components as well as receptors involved in various aspects of leukocyte adhesion. Integrin alpha chain 4 (ITGA4) is assigned to 2q31-q32. Interaction between leukemic-cell VLA-4 and stromal fibronectin is a decisive factor for minimal residual disease of acute myelogenous leukemia.
• Synonyms: Integrin alpha-4, Integrin alpha-IV, VLA-4, VLA4

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hematopoietic cell lineage, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Regulation of actin cytoskeleton