NGF Rabbit Polyclonal Antibody

CAT#: AP23284PU-N

NGF rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat brain tissue lysis using NGFbeta antibody

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  Immunohistochemical analysis of paraffin embedded rat tissue sections (brain), staining NGFbeat in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: At 1.0 μg/ml with the appropriate system to detect NGF beta in cells and tissues.
  Immunohistochemistry on paraffin sections: At 2.0 μg/ml to detect NGF beta in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide mapping human NGF beta
• Specificity: This antibody detects NGF at N-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens nerve growth factor (beta polypeptide) (NGF)
• Database Link:
  Entrez Gene 18049 MouseEntrez Gene 310738 RatEntrez Gene 4803 Human
• Background: Nerve growth factor is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. the nucleotide sequence of human and mouse beta-NGF are very similar. the beta-subunits of nerve growth factor (NGFB) have been assigned to mouse chromosome 3 and human chromosome 1p22. The human gene for the beta subunit of nerve growth factor is located on the proximal short arm of chromosome 1. A mutation in the nerve growth factor beta gene (NGFB) causes loss of pain perception.
• Synonyms: Beta-NGF, NGFB,Beta-nerve growth factor

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Apoptosis, MAPK signaling pathway, Neurotrophin signaling pathway