Chk2 (CHEK2) (C-term) Rabbit Polyclonal Antibody

CAT#: AP23321PU-N

Chk2 (CHEK2) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

CHK2 Polyclonal Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 0.75 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human CHK2
• Specificity: This antibody detects CHK2 (C-term).
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Gene Name: Homo sapiens checkpoint kinase 2 (CHEK2), transcript variant 3
• Database Link:
  Entrez Gene 50883 MouseEntrez Gene 114212 RatEntrez Gene 11200 Human
• Background: CHK2, a protein kinase that is activated in response to DNA damage, is involved in cell cycle arrest.Mapped on 22q12.1, CHK2 has a potential regulatory region rich in SQ and TQ amino acid pairs. It regulates BRCA1 function after DNA damage by phosphorylating serine-988 of BRCA11. Additionally,CHK2 can be modified by phosphorylation and activated in response to ionizing radiation, and can be also modified in response to hydroxyurea treatment2. Furthermore, oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells3. Morever, CHK2 is a tumor suppressor gene conferring predisposition to sarcoma, breast cancer, and brain tumors, and that their observations provided a link between the central role of p53 inactivation in human cancer and the well-defined G2 checkpoint in yeast4. There is a wide expression of small amounts of CHK2 mRNA with larger amounts in human testis, spleen, colon, and peripheral blood leukocytes.
• Synonyms: CHEK2, CHEK-2, CHK-2, RAD53, Cds1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway