AMD1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23364PU-N

AMD1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat kidney tissue lysis using SAMDC antibody

• 

  Immunohistochemical analysis of paraffin-embedded mammary cancer sections, staining SAMDC in cytoplasm, DAB Chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect SAMDC in cells and tissues.
  Immunohistochemistry on paraffin sections: At 0.5-1μg/ml to detect SAMDC in formalin fixed and paraffin embedded tissues.
  Immunohistochemistry on frozen sections: At 0.5-1μg/ml to detect SAMDC in formalin or acetone fixed tissues.
  Immunocytochemistry: At 2-3μg/ml to detect SAMDC in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence at the C-terminal of human SAMDC
• Specificity: This antibody detects AMD1 (C-term). No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens adenosylmethionine decarboxylase 1 (AMD1), transcript variant 1
• Database Link:
  Entrez Gene 11702 MouseEntrez Gene 81640 RatEntrez Gene 262 Human
• Background: S-adenosylmethionine decarboxylase (AdoMet-DC), also known as S-adenosylmethionine decarboxylase proenzyme (SAMDC), is a key enzyme in polyamine biosynthesis. It is localized to chromosome region 6q21-q22. SAMDC has an unusual distribution in polysomes from cells of T lymphocyte origin. It associates predominantly with monosomes and small polysomes with none located in the preribosomal or ribonucleoprotein pool. SAMDC is a critical regulatory enzyme of the polyamine synthetic pathway, and a well-studied drug target. Since SAMDC is a key regulatory enzyme in the synthesis of spermidine and spermine, the marked increase in SAMDC activity in the neonate and the sustained high enzyme levels throughout adulthood, imply a role for these polyamines in both development and mature brain function.
• Synonyms: AdoMetDC, SAMDC

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways