PP2A-alpha (PPP2CA) (N-term) Rabbit Polyclonal Antibody

CAT#: AP23366PU-N

PP2A-alpha (PPP2CA) (N-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat kidney tissue lysis using PP2A antibody

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  Immunohistochemical analysis of paraffin-embedded cancer sections, staining PP2A in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 1-2μg/ml with the appropriate system to detect PP2A in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2μg/ml to detect PP2A in formalin fixed and paraffin embedded tissues. Boiling the sections is required.
  Immunocytochemistry: At 0.5-1μg/ml to detect PP2A in acetone fixed cell. Antigen retrieval by Pepsin and Trypsin is required.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping near the N-terminal of human PP2A
• Specificity: This antibody detects PPP2CA at N-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens protein phosphatase 2 catalytic subunit alpha (PPP2CA)
• Database Link:
  Entrez Gene 19052 MouseEntrez Gene 24672 RatEntrez Gene 5515 Human
• Background: The catalytic subunit of human protein phosphatase 2A (PPP2CA) encodes a 309-amino acid polypeptide.It is localized to chromosome 5. The gene (approximately 30 kbp) is composed of seven exons and six introns. It is predicted to be important for phosphatase enzymatic activity. Methylation of the C-terminal leucine residue (Leu309) of protein serine/threonine phosphatase 2A catalytic subunit (PP2AC) is known to regulate catalytic activity in vitro. Furthermore, PP2A has a fundamental role in cardiac function, and suggests that disturbances in protein phosphatase expression and activity may cause or exacerbate the course of cardiac diseases.
• Synonyms: Protein Phospatase 2A, PP2A-alpha, Replication protein C

Reference Data

• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Protein Pathways: Long-term depression, Oocyte meiosis, TGF-beta signaling pathway, Tight junction, Wnt signaling pathway