Fas Ligand (FASLG) (C-term) Rabbit Polyclonal Antibody

CAT#: AP23382PU-N

Fas Ligand (FASLG) (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry with FAS-L Polyclonal Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1 μg/ml with the appropriate system to detect FAS-L in cells and tissues.
  Immunohistochemistry on paraffin sections: 1-2 μg/ml to detect FAS-L in formalin fixed and paraffin embedded tissues.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a sequence mapping at the C-terminal of human FAS-L
• Specificity: This antibody detects CD178 / Fas Ligand at C-term. No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens Fas ligand (FASLG), transcript variant 1
• Database Link:
  Entrez Gene 25385 RatEntrez Gene 356 Human
• Background: FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M(r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9% at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.
• Synonyms: FASLG, APT1LG1, FASL, TNFSF6, CD95L protein, APTL

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Allograft rejection, Apoptosis, Autoimmune thyroid disease, Cytokine-cytokine receptor interaction, Graft-versus-host disease, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Pathways in cancer, Type I diabetes mellitus