TRIM29 Goat Polyclonal Antibody

CAT#: AP23714PU-N

TRIM29 goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1ug/ml) staining of Peripheral Blood Lymphocytes lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TRIM29 antibody staining of paraffin embedded Human Skin at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

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  Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showiug cytoplasmic and Intermediate filament stainiug. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).

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  Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showiug nuclear stainiug. The nuclear stain is DAPI (blue)

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  Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

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Specifications

Product Data

• Applications: ELISA, FC, IF, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/128000 (Detection Limit).
  Western blot: Approx 37kDa band observed in Human Peripheral Blood Mononucleocytes (PBM) and in lysates of cell lines HeLa. An additional 70kDa band was seen in some PBM lysates (calculated MW of 37.3kDa according to NP_001317311.1 (isoform 2) and 65.8kDa according to NP_036233.2 (isoform 1). Primary incubation 1 hour at room tempetature.
  Recommended concentration: 0.1-1 µg/ml.
  Immunohistochemistry on Paraffin Sections:
  In paraffin embedded Human Skin shows staining of subnuclear structures and cytoplasm in keratinocytes.
  Recommended concentration: 1-2 µg/ml.
  Flow Cytometry analysis of A431 cells.
  Recommended concentration: 10 µg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_036233.2.
• Specificity: This antibody recognizes TRIM29.
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
• Database Link:
  Entrez Gene 23650 Human
• Background: Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.
• Synonyms: ATDC; FLJ36085