TXNDC5 (269-282) Goat Polyclonal Antibody

CAT#: AP23763PU-N

TXNDC5 (269-282) goat polyclonal antibody, Aff - Purified

Product Images

TXNDC5 antibody staining of Human Lymph Nodes lysate at 0.05 ug/ml (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TXNDC5 antibody staining of Mouse Lymph Nodes (A), Stomach (B) and Intestine (C) lysates at 0.1 ug/ml (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: 1/64000 (Detection Limit).
  Western blot: 0.03-0.1 µg/ml. Approx 50+38kDa bands observed in Human Lymph Nodes lysates (calculated MW of 47.6kDa according to NP_110437.2 and of 36.2kDa according to NP_001139021.1). A 48kDa band was observed in Mouse Lymph Node while a 37-38kDa doublet was observed n Mouse Stomach lysates and in Mouse Intestine lysates.
  Immunohistochemistry on Paraffin Sections: 3.75 µg/ml (Human Small Intestine).
• Reactivities: Bovine, Human, Mouse
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_110437.2; NP_001139021.1.
• Specificity: Recognizes TXNDC5 (269-282).
  This antibody is expected to recognize both reported isoforms (NP_110437.2, NP_001139021.1) of TXNDC5.
• Formulation: Tris saline, pH~7.3
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Gene Name: Homo sapiens thioredoxin domain containing 5 (endoplasmic reticulum) (TXNDC5), transcript variant 3
• Database Link:
  Entrez Gene 105245 MouseEntrez Gene 81567 Human
• Background: ERp19 and ERp46 are two newly discovered ER luminal proteins, related to protein disulphide isomerase. Western and Northern blot analyses have revealed that both ERp19 and ERp46 and their respective mRNAs are highly expressed in the liver as compared with other tissues. Both proteins are enriched in purified liver ER vesicles and were localized specifically to the ER in McA-RH7777 hepatocytes. See Knoblach et al. for details.
• Synonyms: ERp46

Reference Data

• Protein Families: Druggable Genome