Cathepsin D (CTSD) Rabbit Polyclonal Antibody

CAT#: AP26341PU-N

Cathepsin D (CTSD) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of Cathepsin D in 3T3 cell lysate (Lane 1) and in Rat kidney tissue lysate (Lane 2).

Specifications

Product Data

• Applications: IP, WB
• Recommended Dilution: Western blot (0.5-4 µg/ml).
  Immunoprecipitation.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide surrounding amino acid 140 of Mouse Cathepsins D
• Specificity: This antibody detects both the 52 kDa proform and the 34 kDa cleaved fragment of Cathepsin D.
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified IgG fraction
  Stabilizer: 30% Glycerol, 0.5% BSA
  Preservative: 0.01% Thimerosal
• Concentration: lot specific
• Purification: Affinity Chromatography
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Homo sapiens cathepsin D (CTSD)
• Database Link:
  Entrez Gene 13033 MouseEntrez Gene 171293 RatEntrez Gene 1509 Human
• Background: Cathepsin D is a normal lysosomal protease that is expressed in all cells. It is an aspartyl protease with a pH optimum in the range of 3-5, and contains two N-linked oligosaccharides. Cathepsin D is synthesized as an inactive 52 kDa pro-enzyme. Activation involves the proteolytic removal of the 43 amino acid profragment and an internal cleavage to generate the two-chain form made up of 34 and 14 kDa subunits. Cathepsin D contains the mannose-6-phosphate lysosomal localization signal that targets the enzyme to the lysosomal compartment where it functions in the normal degradation of proteins. In certain tumor cells, Cathepsin D is abnormally processed and is secreted in its 52 kDa precursor form. Numerous clinical studies as well as in vitro evidence suggest that cathepsin D plays an important role in malignant transformation and may be a useful prognostic indicator for breast cancer.
• Synonyms: CTSD, CPSD

Reference Data

• Protein Families: Druggable Genome, Protease
• Protein Pathways: Lysosome