Tnfrsf1a Rabbit Polyclonal Antibody

CAT#: AP26365PU-N

Tnfrsf1a rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: ELISA, FN, IP, WB
• Recommended Dilution: Flow cytometry: The typical starting working dilution is 1:50.
  Immunoassays.
  Immunoprecipitation.
  Western blot: The typical starting working dilution is 1:50.
  Functional assays.
  Antibody reactivity with cell bound or soluble TNF-RI is not inhibited by high concentrations of mouse TNFa.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity:
  The polyclonal antibody recognizes the extracellular part of the mouse Tumor Necrosis Factor Receptor type 1 (TNF-RI) of the membrane-bound as well as the soluble receptor. Cross reacts with Mouse TNF-RII (problematic in case of TNF-RI knockout studies and when used in high concentrations in functional studies). Shows minimal cross reactivity with Mouse TNF-alpha.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
• Concentration: 0.1 mg/ml
• Purification: Protein A
• Gene Name: tumor necrosis factor receptor superfamily, member 1a
• Database Link:
  Entrez Gene 21937 Mouse
• Background: TNF-RI (~55-60 kDa) is present on most cell types and is considered to play a prominent role in cell stimulation by TNF-alpha. TNF-alpha activates inflammatory responses, induces apoptosis, regulates cellular proliferation, and may even promote cancer progression. The effects of TNF-alpha are mediated by TNF-RI and TNF-RII, which have both distinct and overlapping downstream signaling cascades. Induction of cytotoxicity and other functions are mediated largely via TNF-RI. TNF-RI is equally well activated by both the 17 kDa soluble and 26 kDa membrane-bound form, whereas TNF-RII is efficiently activated only by the membrane bound form of TNF-alpha. TNF-RI signaling is initiated when trimeric TNF-alpha binds TNF-RI receptors. Subsequent TNF-RI trimerization promotes the recruitment of a proximal signaling complex composed of TNF Receptor Associated protein with a Death Domain (TRADD), Receptor Interacting Protein (RIP), cellular Inhibitor of Apoptosis Protein 1 (cIAP1), TNF Receptor Associated Factor 2 (TRAF2), and likely TRAF5. Studies with TNF-RI-deficient mice indicate that TNF-RI mediates most of the proliferation, pro-inflammatory, and apoptosis-activating pathways.
• Synonyms: Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a