ICAM1 Rabbit Polyclonal Antibody

CAT#: AP26388BT-N

ICAM1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, IHC, IP, WB
• Recommended Dilution: Immunohistochemistry on frozen sections.
  Immunoprecipitation.
  Western blot.
  Immuhnoassay.
  The typical starting working dilution for Immunhistology and Western blot is 1:10. For selection of the most useful dilution in a given situation a test staining with cells or tissue known to express the antigen should be performed. To this end a small fresh skin biopsy can be incubated for 4 hours with TNF-alpha (1 ng/ml), IL-1 (100 U/ml) or LPS (1 µg/ml) in tissue culture medium at 37°C.
• Reactivities: Human, Monkey
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Specificity:

  The antibody reacts with both intact human ICAM-1, CD54 and with soluble human ICAM-1.

• Formulation: PBS
  Label: Biotin
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein A
• Conjugation: Biotin
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: intercellular adhesion molecule 1
• Database Link:
  Entrez Gene 3383 Human
• Background: ICAM-1 (intercellular adhesion molecule) has 7 potential N-linked glycosylation sites. It is a single chain glycoprotein of Ig supergene family, present on unstimulated endothelial cells (EC) and on a variety of other cell types including activated fibroblasts, EC, macrophages, and lymphocytes. ICAM-1 mediates cell adhesion by binding to integrins CD11a/CD18 (leukocyte adhesion molecule, LFA-1) and to CD11b/CD18 (Mac-1).
• Synonyms: ICAM-1